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p416 25Q GAL
(Plasmid #1185)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1185 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    htt 25Q
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    250
  • GenBank ID
    NM_002111
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

N terminal region of Ht with polyQ repeat length of 25 fused to GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p416 25Q GAL was a gift from Susan Lindquist (Addgene plasmid # 1185 ; http://n2t.net/addgene:1185 ; RRID:Addgene_1185)
  • For your References section:

    Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins. Krobitsch S, Lindquist S. Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1589-94. 10.1073/pnas.97.4.1589 PubMed 10677504