PurposeExpression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||118727||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)E. coli K12 MG1655
E. coli K12 MG1655 genotype: F- λ- ilvG- rfb-50 rph-1
Integration at HK022 attB: pOSIP-KH-RBS2-dCas9
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FR-E01 was a gift from David Bikard (Addgene plasmid # 118727)
For your References section:Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors. Rousset F, Cui L, Siouve E, Becavin C, Depardieu F, Bikard D. PLoS Genet. 2018 Nov 7;14(11):e1007749. doi: 10.1371/journal.pgen.1007749. eCollection 2018 Nov. 10.1371/journal.pgen.1007749 PubMed 30403660