pBOB-VE-cadherin-tdTomato
(Plasmid #118733)

• Purpose: To express VE-cadherin tagged with tdTomato on C-terminus
• Depositing Lab: John Cooper
• Publication: Cooper Lab plasmids (unpublished)

Backbone

• Vector backbone: pCSC-SP-PW-tdTomato
• Modifications to backbone: tdTomato cDNA was replaced with GFP in pCSC-SP-PW-GFP vector with Age I and Not I
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cadherin 5 (CD144)
• Species: H. sapiens (human)
• Mutation: I503T (Common SNP-see comments)
• Entrez Gene: CDH5 (a.k.a. 7B4, CD144)
• Promoter: CMV
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (unknown if destroyed)
• 3′ cloning site: Age I (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

tdTomato cDNA was replaced with GFP in pCSC-SP-PW-GFP vector with Age I and Not I

I503T is a common SNP that does not affect function. Depositor confirms the expressed protein localizes to cadherin-based cell-cell junctions.