VE-cad-eGFP Donor plasmid
(Plasmid #92309)

• Purpose: Knock eGFP into human VE-cad locus
• Depositing Lab: Sean Palecek
• Publication: Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201. doi: 10.1002/btm2.10062. Epub 2017 May 22.

Backbone

• Vector backbone: Oct4-2A-eGFP backbone
• Backbone size w/o insert (bp): 8563
• Total vector size (bp): 10438
• Modifications to backbone: Left and right arms of Oct4 were replaced with VEcad arms
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VEcad Homologous Recombination Arms
• Alt name: CDH5
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: about 30 a.a. truncated at end of protein
• GenBank ID: 1003
• Entrez Gene: CDH5 (a.k.a. 7B4, CD144)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SbfI, AscI (not destroyed)
• 3′ cloning site: NheI, FseI (not destroyed)
• 5′ sequencing primer: M13 RVS
• 3′ sequencing primer: M13 FWD

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid functions as described in the related publication, but the 5' homology arm is 528bp instead of ~2kbp.