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VE-cad-eGFP Donor plasmid
(Plasmid #92309)


Item Catalog # Description Quantity Price (USD)
Plasmid 92309 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Oct4-2A-eGFP backbone
  • Backbone size w/o insert (bp) 8563
  • Total vector size (bp) 10438
  • Modifications to backbone
    Left and right arms of Oct4 were replaced with VEcad arms
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    VEcad Homologous Recombination Arms
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    about 30 a.a. truncated at end of protein
  • GenBank ID
  • Entrez Gene
    CDH5 (a.k.a. 7B4, CD144)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI, AscI (not destroyed)
  • 3′ cloning site NheI, FseI (not destroyed)
  • 5′ sequencing primer M13 RVS
  • 3′ sequencing primer M13 FWD
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid functions as described in the related publication, but the 5' homology arm is 528bp instead of ~2kbp.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VE-cad-eGFP Donor plasmid was a gift from Sean Palecek (Addgene plasmid # 92309 ; ; RRID:Addgene_92309)
  • For your References section:

    Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells. Bao X, Bhute VJ, Han T, Qian T, Lian X, Palecek SP. Bioeng Transl Med. 2017 Jun;2(2):191-201. doi: 10.1002/btm2.10062. Epub 2017 May 22. 10.1002/btm2.10062 PubMed 29170757