pRIBOZ RF01
(Plasmid #118827)

• Purpose: complete cDNA of rotavirus strain RF gene 1 (VP1) for reverse genetics
• Depositing Lab: Didier Poncet
• Depositing Organization: CNRS UMR 9198, institut de biologie intégrative de la cellule recherche
  Located in France
• Publication: plasmids for bovine rotavirus reverse genetics (unpublished)

Backbone

• Vector backbone: pBluescript II SK(-)
• Backbone size w/o insert (bp): 2851
• Total vector size (bp): 5861
• Modifications to backbone: addition of a T7 promoter, HDV ribozyme and T7 terminator
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RF01 (VP1)
• Alt name: VP1, gene 1, polymerase
• Species: Bovine Rotavirus
• Insert Size (bp): 3302
• Promoter: T7 polymerase

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: AgeI (destroyed during cloning)
• 5′ sequencing primer: M13RP
• 3′ sequencing primer: pBR1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry