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pCMV-OptoTGFBRs
(Plasmid #118942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118942 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 118942-D50 50 μg of DNA in Tris buffer $495
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DNA 118942-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    ptdToamto-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3925
  • Total vector size (bp) 9821
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OptoTGFBRs
  • Alt name
    Lyn-cytoTGFbR1-CIBN-P2A-cytoTGFbR2-PHR-tdTomato
  • Species
    M. musculus (mouse), A. thaliana (mustard weed)
  • GenBank ID
    NM_009370 NM_029575
  • Entrez Gene
    Tgfb1 (a.k.a. TGF-beta1, TGFbeta1, Tgfb, Tgfb-1)
  • Entrez Gene
    Tgfbr2 (a.k.a. 1110020H15Rik, DNIIR, RIIDN, TBR-II, TbetaR-II, TbetaRII)
  • Promoter CMV
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40-pAR
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-OptoTGFBRs was a gift from Won Do Heo (Addgene plasmid # 118942 ; http://n2t.net/addgene:118942 ; RRID:Addgene_118942)

  • For your References section:

    Spatiotemporal Control of TGF-beta Signaling with Light. Li Y, Lee M, Kim N, Wu G, Deng D, Kim JM, Liu X, Heo WD, Zi Z. ACS Synth Biol. 2018 Feb 16;7(2):443-451. doi: 10.1021/acssynbio.7b00225. Epub 2018 Jan 12. 10.1021/acssynbio.7b00225 PubMed 29241005
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