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pGEX-4T-1_RAF1 (51-131)
(Plasmid #119218)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119218 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone size w/o insert (bp) 4969
  • Total vector size (bp) 5212
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Other strains can also be used.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RAF1 aa 51-131
  • Alt name
    RAF1 RBD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    246
  • GenBank ID
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1_RAF1 (51-131) was a gift from Helen Morrison (Addgene plasmid # 119218 ; http://n2t.net/addgene:119218 ; RRID:Addgene_119218)
  • For your References section:

    Inhibition of RAS activation due to a homozygous ezrin variant in patients with profound intellectual disability. Riecken LB, Tawamie H, Dornblut C, Buchert R, Ismayel A, Schulz A, Schumacher J, Sticht H, Pohl KJ, Cui Y, Reis A, Morrison H, Abou Jamra R. Hum Mutat. 2015 Feb;36(2):270-8. doi: 10.1002/humu.22737. 10.1002/humu.22737 PubMed 25504542