BRCA1-plvxt
(Plasmid #119281)

• Purpose: Doxycycline-inducible human BRCA1
• Depositing Lab: Joan Massague
• Publication: Unpublished Joan Massague Lab Plasmids (unpublished)

Backbone

• Vector backbone: pLVX-tight-Puro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 7800
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BRCA1
• Species: H. sapiens (human)
• Insert Size (bp): 5400
• Entrez Gene: BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
• Promoter: TRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: pLVXT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The BRCA1 insert was taken from Addgene plasmid 14999. This plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate.