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pcDNA3.1/CIP2A(1-905) R522D V5 His
(Plasmid #119289)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119289 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5523
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIAA1524
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Mutation
    R522D
  • Entrez Gene
    CIP2A (a.k.a. KIAA1524, NOCIVA, p90)
  • Promoter T7
  • Tag / Fusion Protein
    • V5 His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

522 AGA-> GAT mutation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/CIP2A(1-905) R522D V5 His was a gift from Jukka Westermarck (Addgene plasmid # 119289 ; http://n2t.net/addgene:119289 ; RRID:Addgene_119289)
  • For your References section:

    Oncoprotein CIP2A is stabilized via interaction with tumor suppressor PP2A/B56. Wang J, Okkeri J, Pavic K, Wang Z, Kauko O, Halonen T, Sarek G, Ojala PM, Rao Z, Xu W, Westermarck J. EMBO Rep. 2017 Mar;18(3):437-450. doi: 10.15252/embr.201642788. Epub 2017 Feb 7. 10.15252/embr.201642788 PubMed 28174209