|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11960||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1850
Entrez Genecre (a.k.a. P1_gp003)
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
pBS537 carries a GFPcre fusion gene under the control of a synthetic promoter that can be regulated by a synthetic tet repressor/activator protein. In the presence of doxycycline expression is dramatically turned on to high levels. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS537 tet-hCMV-GFPcre was a gift from Brian Sauer (Addgene plasmid # 11960 ; http://n2t.net/addgene:11960 ; RRID:Addgene_11960)
For your References section:GFPcre fusion vectors with enhanced expression. Le Y, Miller JL, Sauer B. Anal Biochem. 1999 Jun 1. 270(2):334-6. 10.1006/abio.1999.4110 PubMed 10334853