|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11971||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1290
MutationLeucine329 to Proline
Entrez GeneIKBKG (a.k.a. AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3 (Common Sequencing Primers)
Please note that Addgene's quality control sequence confirmed 2 additional amino acids changes in the NEMO ORF - A211T and L301M.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMVTAG-NEMOL329P was a gift from Jon Ashwell (Addgene plasmid # 11971 ; http://n2t.net/addgene:11971 ; RRID:Addgene_11971)
For your References section:NEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Wu CJ, Conze DB, Li T, Srinivasula SM, Ashwell JD. Nat Cell Biol. 2006 Apr . 8(4):398-406. 10.1038/ncb1384 PubMed 16547522