pEGFP-imp α
(Plasmid
#119718)
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PurposeExpresses EGFP-tagged human importinα in mammalian cells
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Depositing Lab
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Depositing OrganizationOsaka Metropolitan University
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119718 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 119718-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 119718-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-ΔC2
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Modifications to backbonepEGFP-delat C2 is constructed by ligation of blunt-ended HindIII site of pEGFP-C1, resulting in the C2-type reading frame
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameimportinα
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SpeciesH. sapiens (human)
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Mutationcontains amino acids 251-529
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Entrez GeneKPNA2 (a.k.a. IPOA1, PTAC58, QIP2, RCH1, SRP1-alpha, SRP1alpha)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
1-kb fragment of the EcoRI-XhoI fragment of DsRed-imp alpha (Addgene plasmid 119679) was cloned into the EcoRI-SalI sites of pEGFP-ΔC2, a frame-shifted derivative of pEGFP-C1.
DNA (Catalog # 119718-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 119718-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-imp α was a gift from Kenji Sugimoto (Addgene plasmid # 119718 ; http://n2t.net/addgene:119718 ; RRID:Addgene_119718) -
For your References section:
A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin alpha as fusion tag. Nakagawa C, Nishimura S, Senda-Murata K, Sugimoto K. Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 10.1271/bbb.110677. Epub 2012 Feb 7. 10.1271/bbb.110677 PubMed 22313767
