pAL612
(Plasmid #119860)

• Purpose: precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine
• Depositing Lab: Linda Randall
• Publication: Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18.

Backbone

• Vector backbone: pET503
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): MM52
• Growth instructions: Change media and shift temperature to 39 degrees with IPTG added to produce the precursor proteins
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
• Alt name: con; ECK0948; tolG; tut
• Species: Escherichia coli str. K-12
• Insert Size (bp): 1041
• Mutation: Cysterine 290 to Serine and Glycine 244 to Cysteine
• GenBank ID: 945571
• Promoter: lac
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: 5' - GGGCGATATTACTGCACC - 3'
• 3′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Donald B. Oliver at Wesleyan University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry