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Co-assembly of SecYEG and SecA fully restores the properties of the native translocon.
Bariya P, Randall LL
J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
119305pAL832precusor outer membrane protein A, ompA, truncated after amino acid 176
119306pAL800precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
119697pAL829precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
119721pAL942precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine
119748pAL950precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
119853pAL928precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C
119860pAL612precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine
119862pAL725precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

Antibodies from Article