Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #119976)


Item Catalog # Description Quantity Price (USD)
Plasmid 119976 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pLenti-sgRNA-lib 2.0 (Addgene plasmid #89638)
  • Backbone manufacturer
    Wensheng Wei Lab
  • Backbone size (bp) 9882
  • Modifications to backbone
    Replaced EGFP with puromycin resistance marker
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-sgRNA(lib)-puro was a gift from Wensheng Wei (Addgene plasmid # 119976 ; ; RRID:Addgene_119976)
  • For your References section:

    Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W. Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283. 10.1038/nbt.4283 PubMed 30395134