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pLenti-sgRNA(lib)-puro
(Plasmid #119976)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119976 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-sgRNA-lib 2.0 (Addgene plasmid #89638)
  • Backbone manufacturer
    Wensheng Wei Lab
  • Backbone size (bp) 9882
  • Modifications to backbone
    Replaced EGFP with puromycin resistance marker
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-sgRNA(lib)-puro was a gift from Wensheng Wei (Addgene plasmid # 119976 ; http://n2t.net/addgene:119976 ; RRID:Addgene_119976)
  • For your References section:

    Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W. Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283. 10.1038/nbt.4283 PubMed 30395134