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pLenti-sgRNA-lib 2.0
(Plasmid #89638)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89638 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLL3.7
  • Backbone manufacturer
    Addgene plasmid #11795
  • Backbone size (bp) 10054
  • Modifications to backbone
    Addition of hU6 promoter and Gateway cloning cassette
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter hU6
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer U6sponge-F (5'-GGACTATCATATGCTTACCGTAACTTGA-3')
  • 3′ sequencing primer StrepPyoCas9-5UTR-F (5'-CGGTGCCACTTTTTCAAGTTG-3')
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-sgRNA-lib 2.0 was a gift from Wensheng Wei (Addgene plasmid # 89638 ; http://n2t.net/addgene:89638 ; RRID:Addgene_89638)
  • For your References section:

    Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W. Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. 10.1038/nbt.3715 PubMed 27798563