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pAM4889 (pCV0001)
(Plasmid #120079)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120079 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 8069
  • Modifications to backbone
    Plasmid assembled from 3 CYANO-VECTOR modular devices: pCVD047 / CV-RSF1010Y25F: The device carries a broad host range replicon derived from RSF1010. A point mutation in mobA prevent self-mobilization. pCVD002 / CV-aadA: The device confers spectinomycin and streptomycin (SpSm) resistance. The stop codon was erased by mistake, another stop codon is however located 15 nt downstream in the transcriptional terminator. This device works perfectly well. pCVD030 / CV-PconII-LTRBS-GFPmut2: Promoter/reporter device where GFPmut2 is driven by a PconII promoter. The RBS and flanking sequences are from the pENTR/SD/D-Topo vector (Life Technologies).
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
  • Species
    Broad range of bacteria
  • Mutation
    mobA Y25F

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM4889 (pCV0001) was a gift from Susan Golden (Addgene plasmid # 120079 ; ; RRID:Addgene_120079)
  • For your References section:

    Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria. Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. Nucleic Acids Res. 2014 Jul 29. pii: gku673. 10.1093/nar/gku673 PubMed 25074377