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Addgene

pRK5 FLAG membrane-bound lipin-1 (TMD)
(Plasmid #120277)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120277 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lipin-1
  • Alt name
    phosphatidate phosphatase 1
  • Alt name
    fatty liver dystrophy
  • Alt name
    PAP1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2900
  • Entrez Gene
    Lpin1 (a.k.a. Lipin1, fld)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • in-frame fusion with the TMD Golgi-localization domain of Syntaxin5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Sabatini Addgene Plasmid #32005

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 FLAG membrane-bound lipin-1 (TMD) was a gift from Sirio Dupont (Addgene plasmid # 120277 ; http://n2t.net/addgene:120277 ; RRID:Addgene_120277)
  • For your References section:

    Extracellular matrix mechanical cues regulate lipid metabolism through Lipin-1 and SREBP. Romani P, Brian I, Santinon G, Pocaterra A, Audano M, Pedretti S, Mathieu S, Forcato M, Bicciato S, Manneville JB, Mitro N, Dupont S. Nat Cell Biol. 2019 Mar;21(3):338-347. doi: 10.1038/s41556-018-0270-5. Epub 2019 Feb 4. 10.1038/s41556-018-0270-5 PubMed 30718857