PurposeExpress 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120548||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameNLS, Sox2 TALE, GCN4
Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
- Promoter EF1α
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Please note that some discrepancies were found between Addgene's quality control result and the depositor's full plasmid sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EF1a-3NLS-Sox2TALE-GCN4 was a gift from Yu Wang (Addgene plasmid # 120548 ; http://n2t.net/addgene:120548 ; RRID:Addgene_120548)
For your References section:Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation. Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y. ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 10.1021/acschembio.7b00606. Epub 2018 Jan 8. 10.1021/acschembio.7b00606 PubMed 29308880