5mCUC1 pBluescript
(Plasmid #12065)

• Depositing Lab: David Bartel
• Publication: Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Backbone

• Vector backbone: pBluescript II SK+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CUC1 mutant
• Alt name: CUC1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 3700
• Mutation: miR-resistant CUC1. Made by introducing five silent mutations within the miR164-complementarity domain of a CUC1 genomic clone.
• Entrez Gene: CUC1 (a.k.a. AT3G15170, ANAC054, ATNAC1, Arabidopsis NAC domain containing protein 54, CUP-SHAPED COTYLEDON1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CUC1 plasmid (addgene # 12064) was used as a starting material. The sequence of the primer pair used for CUC1 mutagenesis is 5mCUC1 forward 5'-CCGATCATCAATACCTTTGCGACGGAGCATGTTTCATGTTTTT CAAATAACTCTGCTGCTCATACCG-3' and 5mCUC1 reverse 5'-CGGTATGAGCAGCAGAGTTATTTGAAAAACATGAAACATGCTCCGTCGCAAAGGTATTGATGATCGG-3'. See "sequence" for the full insert sequence. See article for cloning details.