PurposeMoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120974||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2100
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)4111
- Cloning method Gibson Cloning
- 5′ sequencing primer AAATAGGCGTATCACGAGGCA
- 3′ sequencing primer TTACCGCCTTTGAGTGAGCTG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:C41m was a gift from Richard Murray (Addgene plasmid # 120974 ; http://n2t.net/addgene:120974 ; RRID:Addgene_120974)