PurposeMoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121007||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2100
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemScarlet-I codon optimized with LAA tag
Insert Size (bp)746
/ Fusion Protein
- LAA degradation tag for ssrA (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer AAATAGGCGTATCACGAGGCA
- 3′ sequencing primer TTACCGCCTTTGAGTGAGCTG (Common Sequencing Primers)
Please see Supplemental Documents for annotated Genbank file.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:C103m was a gift from Richard Murray (Addgene plasmid # 121007 ; http://n2t.net/addgene:121007 ; RRID:Addgene_121007)