pCB6-HFE-EGFP
(Plasmid
#12104)
-
Depositing Lab
-
Depositing OrganizationCalifornia Institute of Technology
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 12104-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 12104-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepCB6
-
Backbone manufacturerIra Mellman, Yale University
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameHFE
-
Alt namehemochromatosis protein
-
Alt nameMHC class I-like protein HFE
-
Alt namehereditary hemochromatosis protein HLA-H
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1044
-
GenBank IDQ30201
-
Entrez GeneHFE (a.k.a. HFE1, HH, HLA-H, MVCD7, TFQTL2)
-
Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 12104-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 12104-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCB6-HFE-EGFP was a gift from Pamela Bjorkman (Addgene plasmid # 12104 ; http://n2t.net/addgene:12104 ; RRID:Addgene_12104) -
For your References section:
HFE and transferrin directly compete for transferrin receptor in solution and at the cell surface. Giannetti AM, Bjorkman PJ. J Biol Chem. 2004 Jun 11. 279(24):25866-75. 10.1074/jbc.M401467200 PubMed 15056661