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PurposeExpresses GFP tagged BioID and human FAM21C in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 121046-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 121046-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAcGFP1-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 9687
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameBioID
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Alt nameBirA*
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SpeciesE. Coli
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Insert Size (bp)1116
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MutationR118G (highly promiscuous form)
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GenBank ID
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (not destroyed)
- 3′ cloning site Kpn1 (not destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameFAM21C
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SpeciesH. sapiens (human)
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Insert Size (bp)3963
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GenBank IDNM_004637.5
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Entrez GeneFAM21C
- Promoter CMV
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site kpn1 (not destroyed)
- 3′ cloning site bamh1 (not destroyed)
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 121046-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 121046-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-BioID-FAM21 was a gift from Gia Voeltz (Addgene plasmid # 121046 ; http://n2t.net/addgene:121046 ; RRID:Addgene_121046) -
For your References section:
A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission. Hoyer MJ, Chitwood PJ, Ebmeier CC, Striepen JF, Qi RZ, Old WM, Voeltz GK. Cell. 2018 Sep 20;175(1):254-265.e14. doi: 10.1016/j.cell.2018.08.030. Epub 2018 Sep 13. 10.1016/j.cell.2018.08.030 PubMed 30220460
