|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121061||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepUC19 (modified)
- Backbone size w/o insert (bp) 2600
- Total vector size (bp) 10500
Modifications to backboneAddition of ccdB markers to facilitate homology arm cloning
Vector typeWorm Expression, Cre/Lox, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Turbo
Copy numberHigh Copy
SpeciesC. elegans (nematode), Synthetic
Insert Size (bp)6850
/ Fusion Proteins
- C. elegans codon optimized TagRFP-T
- Biotin acceptor peptide (BioTag)
- auxin-inducible degron (AID*)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid is derived from pDD284 (AddGene plasmid 66825)
Please note: This plasmid contains an eight basepair deletion at end of the homology arm near M13-rev. This deletion is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJW1594 was a gift from Jordan Ward (Addgene plasmid # 121061 ; http://n2t.net/addgene:121061 ; RRID:Addgene_121061)
For your References section:An expanded auxin-inducible degron toolkit for Caenorhabditis elegans. Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD. Genetics, 2021 10.1093/genetics/iyab006