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An expanded auxin-inducible degron toolkit for Caenorhabditis elegans
Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
Genetics, 2021
Article

Plasmids from Article

ID Plasmid Purpose  
121054pJW1583GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
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121055pJW1584YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
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121057pJW1586mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
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121059pJW1592GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
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121061pJW1594TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
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121062pJW1595mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
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154310pJW1347Donor-cassette for SapTrap
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154311pJW1348NT Donor-cassette for SapTrap
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154312pJW1582mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
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154314pJW1659NT Donor-cassette for SapTrap
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154315pJW1759NT Donor-cassette for SapTrap
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154316pJW1787NT Donor-cassette for SapTrap
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154317pJW1788CT Donor-cassette for SapTrap
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154318pJW1805CT Donor-cassette for SapTrap
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154319pJW1806NT Donor-cassette for SapTrap
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154320pJW1816Multi-cassette for SapTrap
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154321pJW1820Multi-cassette for SapTrap
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154322pJW1822Multi-cassette for SapTrap
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154323pJW1827Multi-cassette for SapTrap
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154324pJW1838sgRNA Destination Vector for SapTrap
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154325pJW1839sgRNA Destination Vector for SapTrap
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154326pJW1846Multi-cassette for SapTrap
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154327pJW1849sgRNA Target Vector for CRISPR
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154328pJW1850sgRNA Target Vector for CRISPR
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154329pJW1851sgRNA Target Vector for CRISPR
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154330pJW1882sgRNA Target Vector for CRISPR
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154331pJW1883sgRNA Target Vector for CRISPR
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154332pJW1884sgRNA Target Vector for CRISPR
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154333pJW1886Multi-cassette for SapTrap
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154334pJW2072Crispr repair template: add insertion site specific homology arms by PCR before insertion
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154335pJW2086Crispr repair template: add insertion site specific homology arms by PCR before insertion
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154336pJW2088Crispr repair template: add insertion site specific homology arms by PCR before insertion
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154337pJW1836Promoter reporter
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154338pJW1841Promoter reporter
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154339pJW1947Enhancer reporter
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154340pJW1948Enhancer reporter
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163092pJW1821CRISPR repair template: add insertion site specific homology arms by PCR before insertion
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163093pJW1927CRISPR repair template: add insertion site specific homology arms by PCR before insertion
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163094pJW2098CRISPR repair template: add insertion site specific homology arms by PCR before insertion
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163095pJW2171CRISPR repair template: add insertion site specific homology arms by PCR before insertion
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163096pJW2172CRISPR repair template: add insertion site specific homology arms by PCR before insertion
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