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(Plasmid #1211)


Item Catalog # Description Quantity Price (USD)
Plasmid 1211 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    sup35 NM domain fused with GR
  • Alt name
    Yeast Sup35
  • Alt name
    Rat glucocorticoid receptor
  • Species
    R. norvegicus (rat), S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Entrez Gene
    Nr3c1 (a.k.a. GR, Gcr, Grl)
  • Tag / Fusion Protein
    • GR (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site GR cloned into SacII (5')/SacI site of Lindquist vector 6442 (not destroyed)
  • 3′ cloning site GR in SacII/SacI (3')of Lindquist vector 6442 (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    see comments
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCUP1-NMGR was a gift from Susan Lindquist (Addgene plasmid # 1211 ; ; RRID:Addgene_1211)
  • For your References section:

    Creating a protein-based element of inheritance. Li L, Lindquist S. Science 2000 Jan 28;287(5453):661-4. 10.1126/science.287.5453.661 PubMed 10650001