|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1212||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4848
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameglucocorticoid receptor
SpeciesR. norvegicus (rat)
Insert Size (bp)2840
Entrez GeneNr3c1 (a.k.a. GR, Gcr, Grl)
- Cloning method Restriction Enzyme
- 5′ cloning site NM domain removed from pCUP1-NMGR with BamHI/SacII digest (not destroyed)
- 3′ cloning site NM domain removed from pCUP1-NMGR with BamHI/SacII digest (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bysee comments.
Terms and Licenses
- Not Available to Industry
Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.
Note: Please see Addgene's sequencing result for the GR sequence, which is slightly different from the author's sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCUP1-GR was a gift from Susan Lindquist (Addgene plasmid # 1212 ; http://n2t.net/addgene:1212 ; RRID:Addgene_1212)
For your References section:Creating a protein-based element of inheritance. Li L, Lindquist S. Science 2000 Jan 28;287(5453):661-4. 10.1126/science.287.5453.661 PubMed 10650001