pLHCX-gpASNase1
(Plasmid #121526)

• Purpose: Expresses gpASNase1 in mammalian cells.
• Depositing Labs: Lucas Sullivan, Matthew Vander Heiden
• Publication: Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. doi: 10.1038/s41556-018-0125-0. Epub 2018 Jun 25.

Backbone

• Vector backbone: pLHCX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6813
• Total vector size (bp): 8500
• Vector type: Mammalian Expression, Bacterial Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gpASNase1
• Alt name: guinea pig asparaginase 1
• Alt name: ASPG
• Species: Cavia porcellus (guinea pig)
• Insert Size (bp): 1727
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HINDIII (not destroyed)
• 3′ cloning site: CLAI (not destroyed)
• 5′ sequencing primer: 5' pLNCX Seq Primer
• 3′ sequencing primer: 3' pLNCX Seq Primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry