pCF708
(Plasmid
#121685)
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PurposeEF1a-dTEV-protease-mTagBFP2
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEF1a
- Backbone size w/o insert (bp) 9576
- Total vector size (bp) 10284
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Vector typeLentiviral
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Selectable markersbleoR gene: antibiotic binding protein
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsanother resistance
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTEV protease
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Speciestobacco etch virus
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Insert Size (bp)708
- Promoter EF-1a core promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer 5'-GCCAGCggagaaagtttgttt-3'
- 3′ sequencing primer 5'-GTattcatgagttgagtcgcttcctt-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCF708 was a gift from David Savage (Addgene plasmid # 121685 ; http://n2t.net/addgene:121685 ; RRID:Addgene_121685) -
For your References section:
CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. Oakes BL, Fellmann C, Rishi H, Taylor KL, Ren SM, Nadler DC, Yokoo R, Arkin AP, Doudna JA, Savage DF. Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052. 10.1016/j.cell.2018.11.052 PubMed 30633905
