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pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1
(Plasmid #122083)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122083 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRetroX-pTuner
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5875
  • Total vector size (bp) 9178
  • Vector type
    Mammalian Expression, Bacterial Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DD-linker-M.EcoGII-V5-LaminB1
  • Species
    H. sapiens (human); E.coli
  • Insert Size (bp)
    3303
  • Tag / Fusion Protein
    • V5 tag on Nter of LaminB1 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGACTATATCTCCAG
  • 3′ sequencing primer GTCAAGAAGACAGGGC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    LaminB1 cDNA was obtained from pDEST-LaminB1 vector, originally from Dr. M. Hetzer laboratory
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1 was a gift from Laure Crabbe (Addgene plasmid # 122083 ; http://n2t.net/addgene:122083 ; RRID:Addgene_122083)
  • For your References section:

    MadID, a Versatile Approach to Map Protein-DNA Interactions, Highlights Telomere-Nuclear Envelope Contact Sites in Human Cells. Sobecki M, Souaid C, Boulay J, Guerineau V, Noordermeer D, Crabbe L. Cell Rep. 2018 Dec 4;25(10):2891-2903.e5. doi: 10.1016/j.celrep.2018.11.027. 10.1016/j.celrep.2018.11.027 PubMed 30517874