pS448∙CsR
(Plasmid #122096)

• Purpose: CRISPR/Cas9 counterselection in Gram-negative bacteria
• Depositing Lab: Pablo Ivan Nikel
• Publication: Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13396.

Backbone

• Vector backbone: pSEVA448
• Backbone manufacturer: de Lorenzo´s lab
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cas9
• Species: Streptococcus pyogenes
• GenBank ID: GI:81533697
• Promoter: Pm

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: TCCAGCCTTGCAAGAAGC
• 3′ sequencing primer: GCGGCAACCGAGCGTTC

Gene/Insert 2

• Gene/Insert name: sgRNA
• Alt name: single guide RNA
• Alt name: crRNA - tracrRNA fusion construct
• Promoter: Pem7

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: ACGCTCGGTTGCCGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry