Addgene: Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection.

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Pablo Ivan Nikel
Wirth et al

Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection.
Wirth NT, Kozaeva E, Nikel PI
Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13396. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
122086	pGNW2	suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
122088	pGNW4	suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
122093	pGNW6	suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
122094	pSEVA6213S	constitutive expression of I-SceI for genome engineering in Pseudomonas
122095	pSEVA1213S	constitutive expression of I-SceI for genome engineering in Pseudomonas
122096	pS448∙CsR	CRISPR/Cas9 counterselection in Gram-negative bacteria

Antibodies from Article