pGNW4
(Plasmid #122088)

• Purpose: (Empty Backbone) suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
• Depositing Lab: Pablo Ivan Nikel
• Publication: Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13396.

Backbone

• Vector backbone: pEMG
• Backbone manufacturer: Esteban Martínez-García and Víctor de Lorenzo
• Backbone size (bp): 3168
• Modifications to backbone: introduction of a constitutively expressed monomeric superfolder GFP and exchange of the antibiotic resistance to streptomycin.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5α λpir
• Copy number: Low Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TGTAAAACGACGGCCAGT
• 3′ sequencing primer: ATGACCATGATTACGCCGG

Resource Information

• Supplemental Documents:
  • pGNW4.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry