pRC/RSV-rat Agt
(Plasmid
#122104)
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PurposeExpression of angiotensinogen
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRC/RSV
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5200
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameangiotensinogen
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1400
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Entrez GeneAgt (a.k.a. ANRT, Ang, AngII, PAT)
- Promoter RSV promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRC/RSV-rat Agt was a gift from John Chan (Addgene plasmid # 122104 ; http://n2t.net/addgene:122104 ; RRID:Addgene_122104) -
For your References section:
Essential role(s) of the intrarenal renin-angiotensin system in transforming growth factor-beta1 gene expression and induction of hypertrophy of rat kidney proximal tubular cells in high glucose. Zhang SL, To C, Chen X, Filep JG, Tang SS, Ingelfinger JR, Chan JS. J Am Soc Nephrol. 2002 Feb;13(2):302-12. PubMed 11805157
