Purposeexpresses human flag tagged KLF6 in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49488||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5472
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Alt nameKruppel-like factor 6
SpeciesH. sapiens (human)
Entrez GeneKLF6 (a.k.a. BCD1, CBA1, COPEB, CPBP, GBF, PAC1, ST12, ZF9)
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:flag-hKLF6 (1006) was a gift from Scott Friedman (Addgene plasmid # 49488 ; http://n2t.net/addgene:49488 ; RRID:Addgene_49488)
For your References section:GSK3beta phosphorylation of the KLF6 tumor suppressor promotes its transactivation of p21. Lang UE, Kocabayoglu P, Cheng GZ, Ghiassi-Nejad Z, Munoz U, Vetter D, Eckstein DA, Hannivoort RA, Walsh MJ, Friedman SL. Oncogene. 2012 Oct 22. doi: 10.1038/onc.2012.457. 10.1038/onc.2012.457 PubMed 23085750