Brm WT-Flag pShuttle
(Plasmid
#122105)
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PurposeExpression of full length Brm in mammalian cells
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Depositing Lab
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Depositing OrganizationIndiana University School of Medicine, Indianapolis
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122105 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 122105-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 122105-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepShuttle
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBRM
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Alt nameSMARCA2
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SpeciesH. sapiens (human)
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Insert Size (bp)5000
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Entrez GeneSMARCA2 (a.k.a. BAF190, BIS, BRM, NCBRS, SAMRCA2, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a)
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 122105-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 122105-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Brm WT-Flag pShuttle was a gift from Paul Herring (Addgene plasmid # 122105 ; http://n2t.net/addgene:122105 ; RRID:Addgene_122105) -
For your References section:
The SWI/SNF chromatin remodeling complex regulates myocardin-induced smooth muscle-specific gene expression. Zhou J, Zhang M, Fang H, El-Mounayri O, Rodenberg JM, Imbalzano AN, Herring BP. Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):921-8. doi: 10.1161/ATVBAHA.109.187229. Epub 2009 Apr 2. 10.1161/ATVBAHA.109.187229 PubMed 19342595
Map uploaded by the depositor.
