pD2-dcas9-J23101-GFP
(Plasmid
#122260)
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PurposeConstitutive dCas9 and GFP expression plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBbA2c
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS results identified an insertion sequence in the J23101 promoter driving GFP expression. The depositing lab states that GFP expression should still be constitutive.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pD2-dcas9-J23101-GFP was a gift from Chueh Loo Poh (Addgene plasmid # 122260 ; http://n2t.net/addgene:122260 ; RRID:Addgene_122260) -
For your References section:
Regulating exopolysaccharide gene wcaF allows control of Escherichia coli biofilm formation. Zhang J, Poh CL. Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.1038/s41598-018-31161-7. 10.1038/s41598-018-31161-7 PubMed 30177768
