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pD2-dcas9-J23101-GFP
(Plasmid #122260)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122260 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Synthetic

Cloning Information

Resource Information

Depositor Comments

Addgene NGS results identified an insertion sequence in the J23101 promoter driving GFP expression. The depositing lab states that GFP expression should still be constitutive.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD2-dcas9-J23101-GFP was a gift from Chueh Loo Poh (Addgene plasmid # 122260 ; http://n2t.net/addgene:122260 ; RRID:Addgene_122260)
  • For your References section:

    Regulating exopolysaccharide gene wcaF allows control of Escherichia coli biofilm formation. Zhang J, Poh CL. Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.1038/s41598-018-31161-7. 10.1038/s41598-018-31161-7 PubMed 30177768