pRPLC
(Plasmid
#122261)
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PurposepBRBAD-rbsD-RFP-B0015- B0015-LasR-rbs34-J23108-pBRlasI-rbsD-RFP- B0015
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122261 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBbE8k
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepBRBAD, pBRlasI
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRPLC was a gift from Chueh Loo Poh (Addgene plasmid # 122261 ; http://n2t.net/addgene:122261 ; RRID:Addgene_122261) -
For your References section:
Programming the Dynamic Control of Bacterial Gene Expression with a Chimeric Ligand- and Light-Based Promoter System. Jayaraman P, Yeoh JW, Zhang J, Poh CL. ACS Synth Biol. 2018 Nov 16;7(11):2627-2639. doi: 10.1021/acssynbio.8b00280. Epub 2018 Nov 6. 10.1021/acssynbio.8b00280 PubMed 30359530
