Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

huβB1cry:mAppleCAAX
(Plasmid #122451)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122451 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDestTol2pA2
  • Backbone manufacturer
    Tol2 Kit
  • Backbone size w/o insert (bp) 4445
  • Total vector size (bp) 5540
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human BB1 crystallin:mApple_CAAX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1095
  • GenBank ID
    NG_009826
  • Promoter 300bp Human Beta B1 crystallin promoter

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We obtained the 300bp human BB1 crystallin promoter fragment from Bei-En Chang's laboratory at the National Taiwan University. Hou, H.-H., Kuo, M. Y.-P., Luo, Y.-W. & Chang, B.-E. Recapitulation of human βB1-crystallin promoter activity in transgenic zebrafish. Developmental Dynamics. 235 (2), 435-443, (2006). We obtained pDestTol2pA2 (#394), and p3E-polyA (#302) from the Tol2kit v1.2, and pMEmAppleCAAX (#768) from Tol2kit v2.0 (Kristen Kwan lab University of Utah). Kwan, K. M. et al. The Tol2kit: A multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Developmental Dynamics. 236 (11), 3088-3099, (2007).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

You can access the article associated with this plasmid on JOVE at the following url:https://www.jove.com/video/59528/assessment-zebrafish-lens-nucleus-localization-sutural

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    huβB1cry:mAppleCAAX was a gift from Thomas Schilling (Addgene plasmid # 122451 ; http://n2t.net/addgene:122451 ; RRID:Addgene_122451)
  • For your References section:

    Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity. Vorontsova I, Hall JE, Schilling TF. J Vis Exp. 2019 May 6;(147). doi: 10.3791/59528. 10.3791/59528 PubMed 31107462