pTn7·X-nia
(Plasmid #122592)

• Purpose: Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site
• Depositing Lab: Pablo Ivan Nikel
• Publication: Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13383.

Backbone

• Vector backbone: pTn7-M
• Backbone size w/o insert (bp): 3147
• Total vector size (bp): 5856
• Vector type: Bacterial Expression, Synthetic Biology ; Tn7 genomic integration
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: xylS/Pm->Nia
• Alt name: XylS regulator
• Alt name: nuclear inclusion proteins a
• Species: Pseudomonas putida, peanut stripe potyvirus (PStV)
• Insert Size (bp): 2775
• Promoter: Pm

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: AGGGCGGCGGATTTGTCC
• 3′ sequencing primer: GCGGCAACCGAGCGTTC

Resource Information

• Supplemental Documents:
  • ptn7x-nia.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry