p16-1 hTRF2
(Plasmid #12299)

• Depositing Lab: Titia de Lange
• Publication: Broccoli et al Nat Genet. 1997 Oct . 17(2):231-5.

Backbone

• Vector backbone: N/A
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hTRF2
• Alt name: human TTAGGG repeat binding factor 2
• Alt name: 1641
• Species: H. sapiens (human)
• Insert Size (bp): 2900
• Mutation: A434 deleted; There is a stop codon in the 5'UTR
• Entrez Gene: TERF2 (a.k.a. TRBF2, TRF2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid contains a 3 nucleotide deletion in the hTRF2 sequence when compared to GenBank ID NM_005652.3 which results in the deletion of amino acid A434. The deletion was present in the original construct described in the associated publication below and is not known to affect TRF2 activity.