PurposeG_ subunit for creating C-terminal transplants
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123050||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Growth Strain(s)NEB Turbo Competent E. coli
Copy numberHigh Copy
Gene/Insert nameGpa1 C-terminal Truncation Vector [3-4a]
SpeciesS. cerevisiae (budding yeast)
- Cloning method Gateway Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/10.1101/390559v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pWS936 was a gift from Tom Ellis (Addgene plasmid # 123050 ; http://n2t.net/addgene:123050 ; RRID:Addgene_123050)
For your References section:Engineering a Model Cell for Rational Tuning of GPCR Signaling. Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T. Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. 10.1016/j.cell.2019.02.023 PubMed 30955892