Engineering a Model Cell for Rational Tuning of GPCR Signaling.
Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T
Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PubMed Article
Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T
Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123024 | pWS060 | Pheromone responsive promoter |
| 123025 | pWS053 | Pheromone responsive promoter |
| 123026 | pWS2247 | Tet promoter (TetO(x7)-pLEU2m) |
| 123027 | pWS1078 | Z3E promoter |
| 123028 | pWS480 | LexA UAS |
| 123029 | pWS481 | LexA UAS |
| 123030 | pWS482 | LexA UAS |
| 123031 | pWS483 | LexA UAS |
| 123032 | pWS484 | LexA UAS |
| 123033 | pWS485 | Core promoter |
| 123034 | pWS494 | Core promoter |
| 123035 | pWS491 | Core promoter |
| 123036 | pWS487 | Core promoter |
| 123037 | pWS493 | Core promoter |
| 123038 | pRC246 | Core promoter |
| 123039 | pWS486 | Core promoter |
| 123040 | pWS498 | Core promoter |
| 123041 | pWS496 | Core promoter |
| 123042 | pWS497 | Core promoter |
| 123043 | pWS495 | Core promoter |
| 123044 | pWS067 | _-factor GPCR |
| 123045 | pWS626 | P-factor GPCR |
| 123046 | pWS625 | Adenosine GPCR |
| 123047 | pWS1806 | Melatonin GPCR |
| 123048 | pWS2119 | PbPeptide GPCR |
| 123049 | pWS1037 | G_ subunit |
| 123050 | pWS936 | G_ subunit for creating C-terminal transplants |
| 123051 | pWS939 | Pheromone responsive transcription factor |
| 123052 | pWS926 | LexA bacterial repressor |
| 123053 | pWS931 | TetR bacterial repressor |
| 123054 | pWS930 | Beta-estradiol inducible DNA binding domain |
| 123055 | pWS932 | Pheromone responsive domain |
| 123056 | pWS033 | Green fluorescent protein |
| 123057 | pRC063 | Blue fluorescent protein |
| 123058 | pWS1837 | _-factor pheromone |
| 123059 | pWS788 | _-factor protease |
| 123060 | pWS081 | Regulator of G protein signalling |
| 123061 | pWS509 | Negative regulator of Fus3 |
| 123062 | pWS510 | Negative regulator of Ste12 |
| 123063 | pWS511 | Negative regulator of Ste12 |
| 123064 | pWS627 | G___ bicistronic protein |
| 123065 | pWS1859 | Positive regulator of Ste20 |