zbFGF
(Plasmid #12309)

• Depositing Lab: James Thomson
• Publication: Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

Backbone

• Vector backbone: pET28b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fgf2
• Alt name: bFGF
• Species: D. rerio (zebrafish)
• Insert Size (bp): 465
• Mutation: Ala 11 mutated to Pro, Ser 55 mutated to Asn
• Entrez Gene: fgf2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry