pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123188||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerModified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene
- Backbone size w/o insert (bp) 5874
Vector typePlant Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
Insert Size (bp)2973
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Transient fLUC expression vector for plants; Intron avoids expression in Agrobacteria; Increased virulence in Agrobacteria through VirGN54D in the vector backbone.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS was a gift from Erin Cram & Carolyn Lee-Parsons (Addgene plasmid # 123188 ; http://n2t.net/addgene:123188 ; RRID:Addgene_123188)
For your References section:EASI transformation: An efficient transient expression method for analyzing gene function in Catharanthus roseus seedlings. Mortensen S., Bernal-Franco D., Cole LF., Sathitloetsakun S., Cram EJ., Lee-Parsons CW.. frontiers in Plant Science, 2019