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EASI Transformation: An Efficient Transient Expression Method for Analyzing Gene Function in Catharanthus roseus Seedlings.
Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, Lee-Parsons CWT
Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. eCollection 2019. PubMed Article

Plasmids from Article

ID Plasmid Purpose
123180pSB63 - pL0_fLUC-I (CDS1)Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants
123181pSB123 - pL0_rLUC-I (CDS1)Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants
123182pSB65 - pL0_tGFP-I (CDS1)Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)
123183pSB61 - pL0_V2 (CDS1)Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005
123184pSB139 - pL0_ZCT1 (CDS1)Golden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus roseus; a repressor of the STR1 promoter from C. roseus
123185pSB142 - pL0_ORCA3 (CDS1)Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseus
123186pSB62 - pL0_STR (pro + 5U)Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus
123187pSB90 - pAGM4723_VirGN54DGolden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria
123188pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOSbinary plant vector for transient fLUC (with intron) expression
123189pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOSbinary plant vector for transient fLUC (with intron) expression
123190pSB115 - pL2_pSB90_fLUC-I_tGFP-Ibinary plant vector for transient tGFP (with intron) and fLUC (with intron) expression
123191pSB116 - pL2_pSB90_fLUC-I_P19binary plant vector for transient TBSV P19 and fLUC (with intron) expression
123192pSB117 - pL2_pSB90_fLUC-I_V2binary plant vector for transient TYLCV V2 and fLUC (with intron) expression
123193pSB118 - pL2_pSB90_fLUC-I_HcProbinary plant vector for transient TEV HcPro and fLUC (with intron) expression
123194pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression
123195pSB153 - pL2_pSB90_2x35S::ZCT1::tMASbinary plant vector for transient expression of ZCT1 from Catharanthus roseus
123196pSB160 - pL2_pSB90_2x35S::ORCA3::tMASbinary plant vector for transient expression of ORCA3 from Catharanthus roseus
123197pSB161 - pL2_pSB90_2x35S::GUS::tMASbinary plant vector for transient expression of GUS (with introns)
123198pSB165 - pL1R3_pMAS::rLUC-I::tMASbinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)
123199pSB166 - pL2_pSB90_tMAS::rLUC-I::pMASbinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)
123200pSB167 - pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::rLUC-I::pMASbinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in plants

Antibodies from Article