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pSB90 - pAGM4723_VirGN54D
(Plasmid #123187)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 123187 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAGM4723
  • Backbone manufacturer
    Weber et al., 2011 (DOI:10.1371/journal.pone.0016765)
  • Backbone size (bp) 12773
  • Vector type
    Plant Expression ; Level 2 acceptor vector for expression in plants
  • Selectable markers
    CRED

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VirGN54D from pAD1289 (a gift from A. Das)
  • Species
    Agrobacterium tumefaciens
  • Insert Size (bp)
    1153
  • Mutation
    VirGN54D for increase virulence

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Depositor Comments

Golden Gate (MoClo; Weber et al., 2011) level 2 acceptor vector with Mutation VirGN54D for increased virulence. Increased virulence in Agrobacteria for a high-efficiency gene transfer (increased with and without acetosyringone, compared to original vector control).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB90 - pAGM4723_VirGN54D was a gift from Erin Cram & Carolyn Lee-Parsons (Addgene plasmid # 123187 ; http://n2t.net/addgene:123187 ; RRID:Addgene_123187)
  • For your References section:

    EASI transformation: An efficient transient expression method for analyzing gene function in Catharanthus roseus seedlings. Mortensen S., Bernal-Franco D., Cole LF., Sathitloetsakun S., Cram EJ., Lee-Parsons CW.. frontiers in Plant Science, 2019