pSB90 - pAGM4723_VirGN54D
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123187||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerWeber et al., 2011 (DOI:10.1371/journal.pone.0016765)
- Backbone size (bp) 12773
Vector typePlant Expression ; Level 2 acceptor vector for expression in plants
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameVirGN54D from pAD1289 (a gift from A. Das)
Insert Size (bp)1153
MutationVirGN54D for increase virulence
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Golden Gate (MoClo; Weber et al., 2011) level 2 acceptor vector with Mutation VirGN54D for increased virulence. Increased virulence in Agrobacteria for a high-efficiency gene transfer (increased with and without acetosyringone, compared to original vector control).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSB90 - pAGM4723_VirGN54D was a gift from Erin Cram & Carolyn Lee-Parsons (Addgene plasmid # 123187 ; http://n2t.net/addgene:123187 ; RRID:Addgene_123187)
For your References section:EASI transformation: An efficient transient expression method for analyzing gene function in Catharanthus roseus seedlings. Mortensen S., Bernal-Franco D., Cole LF., Sathitloetsakun S., Cram EJ., Lee-Parsons CW.. frontiers in Plant Science, 2019