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pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS
(Plasmid #123199)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 123199 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSB90
  • Backbone manufacturer
    Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene
  • Backbone size w/o insert (bp) 5851
  • Vector type
    Plant Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tMAS::rLUC-I::pMAS
  • Species
    Synthetic
  • Insert Size (bp)
    1702

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Vector for transient expression of a Renilla luciferase (rLUC, intron for avoiding expression in Agrobacteria). Small insert size and VirGN54D in the vector backbone facilitate strong transient expression in plants.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS was a gift from Erin Cram & Carolyn Lee-Parsons (Addgene plasmid # 123199 ; http://n2t.net/addgene:123199 ; RRID:Addgene_123199)
  • For your References section:

    EASI transformation: An efficient transient expression method for analyzing gene function in Catharanthus roseus seedlings. Mortensen S., Bernal-Franco D., Cole LF., Sathitloetsakun S., Cram EJ., Lee-Parsons CW.. frontiers in Plant Science, 2019