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pLPhygSaCas9
(Plasmid #123262)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 123262 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP72
  • Total vector size (bp) 8995
  • Vector type
    CRISPR ; Leishmania
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Staphylococcus aureus Cas9
  • Alt name
    SaCas9
  • Entrez Gene
    NEWENTRY

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Feng Zhang Addgene plasmid pX601
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPhygSaCas9 was a gift from Greg Matlashewski (Addgene plasmid # 123262 ; http://n2t.net/addgene:123262 ; RRID:Addgene_123262)
  • For your References section:

    Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania. Zhang WW, Matlashewski G. mSphere. 2019 Aug 21;4(4). pii: 4/4/e00408-19. doi: 10.1128/mSphere.00408-19. 10.1128/mSphere.00408-19 PubMed 31434745